The entire treatment, from blood draw, to solution preparation, to injection, takes 30-40 minutes. Before injections are given the skin and underlying tissue is first anesthetized to minimize the discomfort.
The level of discomfort depends on the area being treated. As with any injections, there will be a mild discomfort. The patients will be administered anti-inflammatory medications like Advil, Motrin, ibuprofen, Aleve, Celebrex, and Mobic if necessary.
Anytime a needle is placed anywhere in the body, even getting blood drawn, there is a risk of infection, bleeding, and nerve damage. However, these are very rare. Because PRP uses your own blood, you cannot be allergic to it.
First step in a PRP Therapy injection is to draw the patient’s blood sample to prepare the platelets.
The blood sample is then loaded into a centrifuge and is spun for a certain amount of time to separate the components, mainly Red Blood Cells, Poor Platelet Plasma and Platelet Rich Plasma.
Once you’ve successfully separated the PRP (buffy coat), it can directly be loaded into a syringe for application onto the patient.
In a 20 cc syringe take 2 cc of anticoagulant, then draw 18cc of blood from a subject (final volme : 20 cc).
Inject the drawn blood to Dr.PRP kit through the upper injection port until the blood level reaches the 20cc scale marked on the kit. (For easier injection, tilt the kit)
- Separate the plasma layer and red blood cell (RBC) layer by the first centrifugation. (Prior to centrifugation, balance the weight of kit with counterbalance) - Conditions for the First Centrifugation (RPM) Female - 3,000 RPM 3 minutes Male - 3,000 RPM 4 minutes When fail the seperation - 3,000 RPM 1 minutes *For subjects taking aspirin, antihypertensive drugs, with cardiac disease, anemia, or during menstruation, the centrifugation should be done for 4 min at 1,000 RCF.
After the first centrifugation, identify the separation position of the plasma and the RBC layer. Then adjust the height of the separated boundary to the indicated point by pushing up or pulling down the adjusting knob located at the lower part of the Kit. (If the volume of the plasma layer or the separation is not quite well done, additional centrifugation may be done for about 1-2 min at same RPM)
In order to block the plasma and the RBC layer completely, fasten the adjusting knob (counter-clockwise) located at the lower part of the Kit. Then fasten the valve(counter-clockwise) just above the adjusting knob. Finally fasten the adjusting knob(counter-clockwise) again.
Put the fastened PRP Kit to the centrifuge with counterbalance for the second centrifugation to enrich the concentrated platelet. *Condition for the second centrifugation : 6 min at 3,200 RPM.
Place the PRP Kit in its upright position with care in order to prevent mixing of the separated PRP and PPP. Open the upper sillicone lid on the Kit. Using a 10cc syringe with a long needle (> 5 cm) take out the PPP layer slowly from the upper part leaving 4 cc at the base.
Shake and mix the remaining 4 cc of the PRP layer. Then administrate the PRP.
Click on the button to watch the video so you’ll learn more.
Dr. PRP is registered with the US-FDA as a blood collection tube and is manufactured under ISO-certified process enabling it to be used by the following medical professionals and facilities:
A PRP Therapy For Hair Restoration In Progress. Click on the images to watch the video.
Dr. PRP buffy coat extraction can be done in a single step. For further higher concentration, a second step is needed.